Conventional paternity index and probability of paternity can not be calculated when probe that hybridize to multiple VNTR loci because alleles are not ascertained to specific loci. Therefore, an alternate method based upon the chance of
band-sharing is
used to get paternity index and probability of paternity in this study using multi-locus pV47-2 probe.
From 57 parentage cases of Korean pedigree we found 12.08, 11.86, and 12.31 of average DNA fingerprints present in 57 children, 7 bands are not assignable to neither of the parents and overall mutation rate is 0.0138 per band in an individual.
Average
number of bands shared between nother and father is 1.71, with mean band-sharing coefficiency of 0.1798. All bands present in the child's pattern but absent in the mother's are 5.14 on average as obligatory paternal band, but a child are
transmitted 5,
47 from mother. In 10 non-parentage cases, however, a child shared bands of 1.20 on average with a putative father, whereas shared bands of 5.60 with a biological mother. In the paternity test of a child having 3 bands of obligatory paternal
origin,
paternity index(PI) and probability of paternity(PP) are calculated with the bad-sharing coefficiency of 0.18 as 21.4, and 95.4%, respectively.
These results suggest that RFLP analysis using multi-locus pV47-2 probe could identify with virtual certainty one particular man as the biological father of a child.
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